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scf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec scf
    Scf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scf/MACS+GMP+Recombinant+Human+SCF/us12698315-701-50-51
    Average 93 stars, based on 4 article reviews
    scf - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: CD117 epitope-shielded hematopoietic stem cell transplantation with toxin-free conditioning and in vivo selection ameliorates a β-thalassemia model
    Article Snippet: Low Cytokine media: StemProTM-34 SFM (1x) including nutrient supplements (Cat#10639011, Gibco Life Technologies) supplemented with 20 ng/ml Flt3 ligand (Cat#130-096-479, Miltenyi Biotec), 50 ng/ml TPO (Cat#130-095-752, Miltenyi Biotec), 100 ng/ml SCF (Cat# #130-096-695, Miltenyi Biotec), 500 ng/ml LDL (Cat#2698, STEMCELL Technologies), 1X penicillin-streptomycin (Cat# 15140122, Gibco Life Technologies) and 1X L-glutamine (Cat# 25030081, Gibco Life Technologies).

    Cell Culture:

    Article Title: Oncodevelopmental plasticity of the skeleton in myeloid neoplasms
    Article Snippet: In brief, for mesoderm induction, cell aggregates were cultured in phase I medium composed of STEMDiff APEL2 medium (StemCell Technologies, #05275) supplemented with 50ng/mL FGF2 (PeproTech, #100-18B) and 50ng/mL BMP4 (Miltenyi Biotec, #130-111-168), 3mM CHIR99021 (Merck, #SML1046) and 25ng/mL VEGF (Miltenyi Biotec, #130-109-385). .. For hematopoietic and vascular commitment, organoids were cultured in phase II medium consisting of 10ng/mL SCF (Miltenyi Biotec, #130-096-692), 25ng/mL FLT3L (Peprotech, #300-19-250), 25ng/mL VEGF-A (Miltenyi Biotec, #130-109-385). .. From day 5 onward, organoids were embedded in a collagen-based hydrogel containing 60% collagen (collagen type I (Corning, #354236), collagen type IV (Cell Systems, #5022)) and 40% matrigel (Corning, #354230) and cultured under vascular sprouting conditions using phase III media supplemented with additional 5% KO serum replacement (Gibco, #10828010), 5U/mL heparin (Merck, #H3149), 50ng/mL FLT3L (PeproTech, #300-19-250), 20ng/mL IL-3 (ImmunoTools, #11340037), 20ng/mL hyper–IL-6 (Stem Cell Technologies, #78050), 50ng/mL M-CSF (PeproTech, #300-25), 50ng/mL TPO (Peprotech, #315-14) and 50ng/mL EPO (Immunotools, #11344795).

    Incubation:

    Article Title: Baboon endogenous retrovirus (ERV) envelope pseudotyped lentiviral vectors outperform human ERV lentivectors for transduction of T, B, NK and HSPCs
    Article Snippet: Purity of isolated B was monitored using anti-hCD19-APC antibodies (cat #130-113-647, Miltenyi Biotec), and was analyzed by flow cytometry (MACSQuant VYB, Milteny Biotec). .. Human CD34 + -cells were incubated for 14 h or 18–24 h (as indicated) in 24-well plates in serum-free CellGro medium (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with human recombinant cytokines purchased from Miltenyi Biotec (Germany): SCF (100 ng/ml; # 130-096-692), TPO, (20 ng/ml; # 130-096-692), Flt3-L, (100 ng/ml; 130-096-474). .. Transductions were performed on retronectin coated plates according to manufacturer’s instructions (Takara Bio, Mountain View, CA, USA).

    Recombinant:

    Article Title: Baboon endogenous retrovirus (ERV) envelope pseudotyped lentiviral vectors outperform human ERV lentivectors for transduction of T, B, NK and HSPCs
    Article Snippet: Purity of isolated B was monitored using anti-hCD19-APC antibodies (cat #130-113-647, Miltenyi Biotec), and was analyzed by flow cytometry (MACSQuant VYB, Milteny Biotec). .. Human CD34 + -cells were incubated for 14 h or 18–24 h (as indicated) in 24-well plates in serum-free CellGro medium (Sartorius CellGenix GmbH, Freiburg, Germany) supplemented with human recombinant cytokines purchased from Miltenyi Biotec (Germany): SCF (100 ng/ml; # 130-096-692), TPO, (20 ng/ml; # 130-096-692), Flt3-L, (100 ng/ml; 130-096-474). .. Transductions were performed on retronectin coated plates according to manufacturer’s instructions (Takara Bio, Mountain View, CA, USA).



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